Institut du Fer-à-Moulin

Unité 839

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Imaging facility

The IFM Imaging Facility

Light Microscopy

Electron Microscopy

What is the IFM Imaging Facility?

The Cell and Tissue Imaging Facility of Institut du Fer à Moulin provides IFM scientists and external users, access to up-to-date imaging technologies and scientific and technical expertise for their research projects. It is part of the Imaging facility of Institut Fédératif de recherche 83 of Integrative Biology (INSERM, CNRS, UPMC). It is also part of the UPMC Imaging facilities network.

Access, Reservation, and Fees

The conditions for access depend on the level of training of the users and the type of service they need. Any user must first contact the Person in charge to register: Light Microscopy or Electron Microscopy.

Registered users can make online reservations at the IFR83 web site: résa-IFR. See the fees list.

Imaging facility staff

Scientific coordination

- René-Marc MEGE (DR2, CNRS)

RM Mège is coordinator of the UPMC Imaging facilities network and team leader at the IFM.

Advanced light microscopy and image analysis

- Theano EIRINOPOULOU (IR1, Inserm) Person in charge

- Mythili SAVARIRADJANE (TR, UPMC)

Electron microscopy

- Carmen CIFUENTES-DIAZ (IR1, Inserm) Person in charge

- Jean-Paul RIO (IE, Inserm)

- Jocelyne BUREAU (IE, Inserm)

Publications

- Cifuentes-Diaz C, Dubourg O, Irinopoulou T, Vigny M, Lachkar S, L Decker, Charnay P, Denisenko N, Maisonobe T, Léger JM, Viala K, Hauw JJ, Girault JA. Nodes of Ranvier and paranodes in chronic acquired neuropathies. PLoS One 2011, in press.

- Azeredo da Silveira S, Schneider BL, Cifuentes-Diaz C, Sage D, Abbas-Terki T, Iwatsubo T, Unser M, Aebischer P. Phosphorylation does not prompt, nor prevent, the formation of alpha-synuclein toxic species in a rat model of Parkinson's disease. Hum Mol Genet. 2009 18:872-87.

- Bertran-Gonzalez J, Håkansson K, Borgkvist A, Irinopoulou T, Brami-Cherrier K, Usiello A, Greengard P, Hervé D, Girault JA, Valjent E, Fisone G. Histone H3 phosphorylation is under the opposite tonic control of dopamine D2 and adenosine A2A receptors in striatopallidal neurons. Neuropsychopharmacology. 2009 34:1710-20.

- Degoutin J, Brunet-de Carvalho N, Cifuentes-Diaz C, Vigny M. ALK (Anaplastic Lymphoma Kinase) expression in DRG neurons and its involvement in neuron-Schwann cells interaction. Eur J Neurosci, 2009 29:275-86.

- Faklaris O, Joshi V, Irinopoulou T, Tauc P, Sennour M, Girard H, Gesset C, Arnault JC, Thorel A, Boudou JP, Curmi PA, Treussart F. Photoluminescent diamond nanoparticles for cell labeling: study of the uptake mechanism in mammalian cells. ACS Nano. 2009 3:3955-62.

- Martrille L, Irinopoulou T, Bruneval P, Baccino E, Fornes P. Age at death estimation in adults by computer-assisted histomorphometry of decalcified femur cortex.J Forensic Sci. 2009 54:1231-7.

- Biondi O, Grondard C, Lécolle S, Deforges S, Pariset C, Lopes P, Cifuentes-Diaz C, Li H, della Gaspera B, Chanoine C, Charbonnier F. Exercise-induced activation of NMDA receptor promotes motor unit development and survival in a type 2 spinal muscular atrophy model mouse. J Neurosci. 2008 28:953-62.

- Denisenko N, Cifuentes-Diaz C, Irinopoulou T, Carnaud M, Benoit E, Niwa-Kawakita M, Chareyre F, Giovannini M, Girault JA, Goutebroze L. Tumor suppressor schwannomin/merlin is critical for the organization of Schwann cell contacts in peripheral nerves. J Neurosci. 2008 28:10472-81.

- Martin PM, Carnaud M, Garcia del Caño G, Irondelle M, Irinopoulou T, Girault JA, Dargent B, Goutebroze L. Schwannomin-interacting protein-1 isoform IQCJ-SCHIP-1 is a late component of nodes of Ranvier and axon initial segments. J Neurosci. 2008 28:6111-7.

- Martin PM, Cifuentes-Diaz C, Garcia M, Goutebroze L, Girault JA. [Axon and Schwann cells... so far away, so close.] Rev Neurol (Paris). 2008 164:1057-62. Review French.

- Petrakis S, Irinopoulou T, Panagiotidis CH, Engelstein R, Lindstrom J, Orr-Urtreger A, Gabizon R, Grigoriadis N, Sklaviadis T. Cellular prion protein co-localizes with nAChR beta4 subunit in brain and gastrointestinal tract. Eur J Neurosci. 2008 27:612-20.

- Vial S, Mansuy C, Sagan S, Irinopoulou T, Burlina F, Boudou JP, Chassaing G, Lavielle S. Peptide-grafted nanodiamonds: preparation, cytotoxicity and uptake in cells. Chembiochem. 2008 9:2113-9.

- Piochon C, Irinopoulou T, Brusciano D, Bailly Y, Mariani J, Levenes C. NMDA receptor contribution to the climbing fiber response in the adult mouse Purkinje cell. J Neurosci. 2007 27:10797-809.

- Proux-Gillardeaux V, Raposo G, Irinopoulou T, Galli T. Expression of the Longin domain of TI-VAMP impairs lysosomal secretion and epithelial cell migration. Biol Cell. 2007 99:261-71.

- Dasgupta S, Repessé Y, Bayry J, Navarrete AM, Wootla B, Delignat S, Irinopoulou T, Kamaté C, Saint-Remy JM, Jacquemin M, Lenting PJ, Borel-Derlon A, Kaveri SV, Lacroix-Desmazes S. VWF protects FVIII from endocytosis by dendritic cells and subsequent presentation to immune effectors.Blood. 2007 109:610-2.

- Alberts P, Rudge R, Iri oulou T, Danglot L, Gauthier-Rouvière C, Galli T. Cdc42 and actin control polarized expression of TI-VAMP vesicles to neuronal growth cones and their fusion with the plasma membrane. Mol Biol Cell. 2006 17:1194-203.

- Proux-Gillardeaux V, Gavard J, Irinopoulou T, Mège RM, Galli T. Tetanus neurotoxin-mediated cleavage of cellubrevin impairs epithelial cell migration and integrin-dependent cell adhesion. Proc Natl Acad Sci USA. 2005 102:6362-7.

- Corvol JC, Valjent E, Toutant M, Enslen H, Irinopoulou T, Lev S, Hervé D, Girault JA. Depolarization activates ERK and proline-rich tyrosine kinase 2 (PYK2) independently in different cellular compartments in hippocampal slices. J Biol Chem. 2005 280:660-8.

Publié le dimanche 06 février 2011

FLASH

For more see "Publications"

Some recent publications from IFM groups

Faure C, Ramos M, Girault JA. Pyk2 cytonuclear localization: mechanisms and regulation by serine dephosphorylation. (2013) Cell Mol Life Sci 70:137-52.

Alcacer C, Santini E, Valjent E, Gaven F, Girault JA, Herve D. Galpha(olf) mutation allows parsing the role of cAMP-dependent and extracellular signal-regulated kinase-dependent signaling in L-3,4-dihydroxyphenylalanine-induced dyskinesia. (2012) J Neurosci 32:5900-10.

Baudoin JP, Viou L, Launay PS, Luccardini C, Espeso Gil S, Kiyasova V, Irinopoulou T, Alvarez C, Rio JP, Boudier T, Lechaire JP, Kessaris N, Spassky N, Metin C. Tangentially Migrating Neurons Assemble a Primary Cilium that Promotes Their Reorientation to the Cortical Plate. (2012) Neuron 76:1108-22.

Bazelot M, Simonnet J, Dinocourt C, Bruel-Jungerman E, Miles R, Fricker D, Francis F. Cellular anatomy, physiology and epileptiform activity in the CA3 region of Dcx knockout mice: a neuronal lamination defect and its consequences. (2012) Eur J Neurosci 35:244-56.

Bellone C, Mameli M. mGluR-Dependent Synaptic Plasticity in Drug-Seeking. (2012) Front Pharmacol 3:159.

Bouthour W, Leroy F, Emmanuelli C, Carnaud M, Dahan M, Poncer JC, Levi S. A human mutation in Gabrg2 associated with generalized epilepsy alters the membrane dynamics of GABAA receptors. (2012) Cereb Cortex 22:1542-53.

Cabezas C, Irinopoulou T, Gauvain G, Poncer JC. Presynaptic But Not Postsynaptic GABA Signaling at Unitary Mossy Fiber Synapses. (2012) J Neurosci 32:11835-40.

Chamma I, Chevy Q, Poncer JC, Levi S. Role of the neuronal K-Cl co-transporter KCC2 in inhibitory and excitatory neurotransmission. (2012) Front Cell Neurosci 6:5.

Danglot L, Freret T, Le Roux N, Neme NN, Burgo A, Hyenne V, Roumier A, Contremoulins V, Dauphin F, Bizot JC, Vodjdani G, Gaspar P, Boulouard M, Poncer JC, Galli T, Simmler MC. Vezatin is essential for dendritic spine morphogenesis and functional synaptic maturation. (2012) J Neurosci 32:9007-22.

Despeaux M, Chicanne G, Rouer E, De Toni-Costes F, Bertrand J, Mansat-De Mas V, Vergnolle N, Eaves C, Payrastre B, Girault JA, Racaud-Sultan C. Focal adhesion kinase splice variants maintain primitive acute myeloid leukemia cells through altered wnt signaling. (2012) Stem Cells 30:1597-610.

Devaux S, Poulain FE, Devignot V, Lachkar S, Irinopoulou T, Sobel A. Specific serine-proline phosphorylation and glycogen synthase kinase 3beta-directed subcellular targeting of stathmin 3/Sclip in neurons. (2012) J Biol Chem 287:22341-53.

Diaz SL, Doly S, Narboux-Neme N, Fernandez S, Mazot P, Banas SM, Boutourlinsky K, Moutkine I, Belmer A, Roumier A, Maroteaux L. 5-HT(2B) receptors are required for serotonin-selective antidepressant actions. (2012) Mol Psychiatry 17:154-63.

Erzurumlu RS, Gaspar P. Development and critical period plasticity of the barrel cortex. (2012) Eur J Neurosci 35:1540-53.

Fernandez SP, Gaspar P. Investigating anxiety and depressive-like phenotypes in genetic mouse models of serotonin depletion. (2012) Neuropharmacology 62:144-54.

French CA, Jin X, Campbell TG, Gerfen E, Groszer M, Fisher SE, Costa RM. An aetiological Foxp2 mutation causes aberrant striatal activity and alters plasticity during skill learning. (2012) Mol Psychiatry 17:1077-85.

Fuchs T, Saunders-Pullman R, Masuho I, Luciano MS, Raymond D, Factor S, Lang AE, Liang TW, Trosch RM, White S, Ainehsazan E, Herve D, Sharma N, Ehrlich ME, Martemyanov KA, Bressman SB, Ozelius LJ. Mutations in GNAL cause primary torsion dystonia. (2012) Nat Genet 45:88-92.

Gangarossa G, Longueville S, De Bundel D, Perroy J, Herve D, Girault JA, Valjent E. Characterization of dopamine D1 and D2 receptor-expressing neurons in the mouse hippocampus. (2012) Hippocampus 22:2199-207.

Gaspar P, Lillesaar C. Probing the diversity of serotonin neurons. (2012) Philos Trans R Soc Lond B Biol Sci 367:2382-94.

Girault JA. Integrating neurotransmission in striatal medium spiny neurons. (2012) Adv Exp Med Biol 970:407-29.

Girault JA. Signaling in striatal neurons: the phosphoproteins of reward, addiction, and dyskinesia. (2012) Prog Mol Biol Transl Sci 106:33-62.

Godin JD, Thomas N, Laguesse S, Malinouskaya L, Close P, Malaise O, Purnelle A, Raineteau O, Campbell K, Fero M, Moonen G, Malgrange B, Chariot A, Metin C, Besson A, Nguyen L. p27(Kip1) is a microtubule-associated protein that promotes microtubule polymerization during neuron migration. (2012) Dev Cell 23:729-44.

Goebbels S, Oltrogge JH, Wolfer S, Wieser GL, Nientiedt T, Pieper A, Ruhwedel T, Groszer M, Sereda MW, Nave KA. Genetic disruption of Pten in a novel mouse model of tomaculous neuropathy. (2012) EMBO Mol Med 4:486-99.

Launay JM, Herve P, Callebert J, Mallat Z, Collet C, Doly S, Belmer A, Diaz SL, Hatia S, Cote F, Humbert M, Maroteaux L. Serotonin 5-HT2B receptors are required for bone-marrow contribution to pulmonary arterial hypertension. (2012) Blood 119:1772-80.

Lelievre EC, Plestant C, Boscher C, Wolff E, Mege RM, Birbes H. N-cadherin mediates neuronal cell survival through Bim down-regulation. (2012) PLoS One 7:e33206.

Manceau V, Kremmer E, Nabel EG, Maucuer A. The protein kinase KIS impacts gene expression during development and fear conditioning in adults. (2012) PLoS One 7(8):e43946.

Maroteaux M, Mameli M. Cocaine evokes projection-specific synaptic plasticity of lateral habenula neurons. (2012) J Neurosci 32:12641-6.

Mazot P, Cazes A, Dingli F, Degoutin J, Irinopoulou T, Boutterin MC, Lombard B, Loew D, Hallberg B, Palmer RH, Delattre O, Janoueix-Lerosey I, Vigny M. Internalization and down-regulation of the ALK receptor in neuroblastoma cell lines upon monoclonal antibodies treatment. (2012) PLoS One 7:e33581.

Meffre J, Chaumont-Dubel S, Mannoury la Cour C, Loiseau F, Watson DJ, Dekeyne A, Seveno M, Rivet JM, Gaven F, Deleris P, Herve D, Fone KC, Bockaert J, Millan MJ, Marin P. 5-HT(6) receptor recruitment of mTOR as a mechanism for perturbed cognition in schizophrenia. (2012) EMBO Mol Med 4:1043-56.

Mouton-Liger F, Paquet C, Dumurgier J, Bouras C, Pradier L, Gray F, Hugon J. Oxidative stress increases BACE1 protein levels through activation of the PKR-eIF2alpha pathway. (2012) Biochim Biophys Acta 1822:885-96.

Mouton-Liger F, Paquet C, Dumurgier J, Lapalus P, Gray F, Laplanche JL, Hugon J. Increased cerebrospinal fluid levels of double-stranded RNA-dependant protein kinase in Alzheimer's disease. (2012) Biol Psychiatry 71:829-35.

Narboux-Neme N, Evrard A, Ferezou I, Erzurumlu RS, Kaeser PS, Laine J, Rossier J, Ropert N, Sudhof TC, Gaspar P. Neurotransmitter release at the thalamocortical synapse instructs barrel formation but not axon patterning in the somatosensory cortex. (2012) J Neurosci 32:6183-96.

Paquet C, Mouton-Liger F, Meurs EF, Mazot P, Bouras C, Pradier L, Gray F, Hugon J. The PKR activator PACT is induced by Abeta: involvement in Alzheimer's disease. (2012) Brain Pathol 22:219-29.

Rebsam A, Bhansali P, Mason CA. Eye-specific projections of retinogeniculate axons are altered in albino mice. (2012) J Neurosci 32:4821-6.

Renner M, Schweizer C, Bannai H, Triller A, Levi S. Diffusion barriers constrain receptors at synapses. (2012) PLoS One 7:e43032.

Stacey D, et al. RASGRF2 regulates alcohol-induced reinforcement by influencing mesolimbic dopamine neuron activity and dopamine release. (2012) Proc Natl Acad Sci U S A 109:21128-21133.

Voyatzis S, Muzerelle A, Gaspar P, Nicol X. Modeling activity and target-dependent developmental cell death of mouse retinal ganglion cells ex vivo. (2012) PLoS One 7:e31105.